Tissue plasminogen activator (tPA, Alteplase) catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. Its fibrinolysis properties makes it clinically useful for treatment of treatment of myocardial infarction with ST-elevation (STEMI), acute ischemic emboilc or thrombotic stroke (AIS), acute massive pulmonary embolism, and central venous access devices (CVAD). Alteplase is produced by Genentech, and is manufactured using recombinant biotechnology techniques. Other r-tPA medications are Reteplase and Tenecteplase.

Unfortunately these medications are not used daily, and often only in emergent situations. For facilities without 24-hour pharmacy coverage, it is often left to the nursing staff or physician to prepare the medication. The preparation instructions included in the kit are complicated and long, which make it difficult to go through during stressful situations. It is beneficial for you to be familiar with the procedure before the need arises. In this tutorial I will walk you through the mixing procedure.

Step 1

When you the open the kit you will find a vial of sterile water and a sterile vial of powdered medication (blue cap).