Tissue plasminogen activator (tPA, Alteplase) catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. Its fibrinolysis properties makes it clinically useful for treatment of treatment of myocardial infarction with ST-elevation (STEMI), acute ischemic emboilc or thrombotic stroke (AIS), acute massive pulmonary embolism, and central venous access devices (CVAD). Alteplase is produced by Genentech, and is manufactured using recombinant biotechnology techniques. Other r-tPA medications are Reteplase and Tenecteplase.

Unfortunately these medications are not used daily, and often only in emergent situations. For facilities without 24-hour pharmacy coverage, it is often left to the nursing staff or physician to prepare the medication. The preparation instructions included in the kit are complicated and long, which make it difficult to go through during stressful situations. It is beneficial for you to be familiar with the procedure before the need arises. In this tutorial I will walk you through the mixing procedure.

Step 1

When you the open the kit you will find a vial of sterile water and a sterile vial of powdered medication (blue cap).


Genentech Alteplase tPA Box

Opened box of Alteplase showing tPA vial and saline vial

Notice how the tPA is in powder form.


In the box you will also find the package insert (which is worth reading when you have a chance) and a dual sided spike which is wrapped in sterile packaging. The spike is the same on both sides, and has a dual channel so that air and fluid can flow in opposite directions.


Step 2

Remove the cap of the water vial sterilely. Unwrap the spike and hold it under sterile condiions. Holding the vial in the upright position, spike the vial.


Step 3

Continuing under sterile conditions, remove the cap from the powdered tPA vial. Hold the water vial firmly in the upright position. With your other hand hold the tPA upside down, and lower the vial down onto the spike.


With both vials firmly spiked, invert both vials together. At this point, the water will on top and will flow from the water vial into the tPA vial. Hold the vials firmly, because the high center of gravity will make them unsteady. This process will take about 1 minute. Do not agitate the vials duri